Substrate Specificity of Pregastric Esterase from the Calf
نویسندگان
چکیده
منابع مشابه
Inhibition studies on calf pregastric esterase: the enzyme has no functional thiol group.
Pregastric esterase (PGE) (EC 3.1.1.3) was purified to homogeneity from calf pharyngeal tissue. The enzyme had an apparent molecular mass of 50 kDa, as determined by SDS/PAGE. The serine-binding reagent diethyl p-nitrophenyl phosphate was a potent inhibitor of PGE. This is in accordance with the claim that a functional serine residue is necessary for the lipolytic activity of lipases. PGE was n...
متن کاملEsterase substrate specificity in the testis of the growing mouse
The age distribution of esterases in the interstitial cell of the mouse testis was studied between birth and the end of the tenth week of postnatal life by using the substrates O-acetyl-s-bromoindoxyl, a-naphthyl acetate, naphthol AS acetate, naphthol AS-LC acetate, and naphthol AS-D acetate. With indoxyl acetate, esterase activity is prominent at birth and wanes over the first 2 weeks of postn...
متن کاملPlant sterol and stanol substrate specificity of pancreatic cholesterol esterase.
Consumption of plant sterols or stanols (collectively referred to as phytosterols) and their esters results in decreased low-density lipoprotein cholesterol, which is associated with decreased atherosclerotic risk. The mechanisms by which phytosterols impart their effects, however, are incompletely characterized. The objective of the present study is to determine if pancreatic cholesterol ester...
متن کاملMolecular determinants of substrate specificity in the feruloyl esterase module of xylanase 10B from Clostridium thermocellum.
Feruloyl esterases play a key role in the degradation of the intricate structure of the plant cell wall by hydrolysing the ferulate ester groups involved in the cross-linking between hemicelluloses and between hemicellulose and lignin. The structure of the feruloyl esterase module of Clostridium thermocellum cellulosomal xylanase 10B has been reported previously. It displays the alpha/beta hydr...
متن کاملPig liver esterase catalyzed hydrolysis: substrate specificity and stereoselectivity
In order to gain a more detailed insight into the relationship between substrate structure and the stereoselectivity of the enzyme pig liver esterase (E.C. 3.1.1 .I .) a large series of mainly meso and prochiral diesters with an open chain or a cyclic structure has been studied and evaluated. Results obtained with 3substituted cyclopropane-l,2-dicarboxylates are incompatible with the three-dime...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1961
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(61)90060-1